Repeated-Batch and Continuous Production of L-Lactic Acid by Rhizopus oryzae Immobilized in Calcium Alginat Beads:ReactorPerformance and Kinetic Model

Repeated-batch and continuous production of L-lactic acid by immobilized Rhizopusoryzae with calcium alginate entrapment method in a three-phase fluidized-bed bioreactor was stud-ied.The operation conditions were optimized.The productivity based on total reactor volume wasabout 3 times higher than that with free cells in a traditional stirred tank bioreactor.A mathemat-ical model was proposed and the model predictions were in good agreement with the experimentaldat.

Repeated-batch and continuous production of L-lactic acid by immobilized Rhizopus oryzae with calcium alginate entrapment method in a three-phase fluidized-bed bioreactor was studied. The operation conditions were optimized. The productivity based on total reactor volume was about 3 times higher than that with free cells in a traditional stirred tank bioreactor. A mathematical model was proposed and the model predictions were in good agreement with the experimental data.