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Evaluation of The Interaction between Netropsin and Double Stranded DNA by Capillary Zone Electrophoresis

Evaluation of The Interaction between Netropsin and Double Stranded DNA by Capillary Zone Electrophoresis
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摘要 Capillary zone electrophoresis (CZE) was applied to study the interaction between netropsin and a 14mer double stranded DNA (dsDNA). The binding constant of this interaction calculated from Scatchard plot was (1.070.10)×105 (mol/L)-1. The binding stoichiometry was 1:1. The use of polyacrylamide coated capillary showed better effect in the analysis of DNA than noncoated capillary. Capillary zone electrophoresis (CZE) was applied to study the interaction between netropsin and a 14mer double stranded DNA (dsDNA). The binding constant of this interaction calculated from Scatchard plot was (1.070.10)×105 (mol/L)-1. The binding stoichiometry was 1:1. The use of polyacrylamide coated capillary showed better effect in the analysis of DNA than noncoated capillary.
作者 Xin Ya HE Da Zhi LI Ai Ye LIANG Bing Cheng LIN
机构地区 Dalian Institute of Chemical Physics
出处 《Chinese Chemical Letters》 SCIE CAS CSCD 2003年第3期304-307,共4页 中国化学快报(英文版)
基金 This project is supported by NNSFC (grant number 20035010). The authors gratefully acknowledge this financial support.
关键词 INTERACTION netropsin DNA capillary zone electrophoresis. Interaction netropsin DNA capillary zone electrophoresis.
分类号 O658.9 [理学—分析化学]
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Chinese Chemical Letters
2003年 第3期

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