双波长比值光谱法测定复方乙酰水杨酸片中三组分含量

Determination of Three Components in Compound Acetylsalicylic Acid Tablet by Dual-Wavelength Ratio Spectrometry

应用双波长比值光谱法测定了复方乙酰水杨酸片中三组分的含量。依据复方乙酰水杨酸片中三组分的比值光谱特征 ,选择 2 13,2 2 7,2 4 5和 2 6 5nm作为测定三个组分的波长。结果显示乙酰水杨酸在 5～ 2 0μg·mL- 1 ,非那西丁在 2～ 10 μg·mL- 1 ,咖啡因在 2～ 2 0 μg·mL- 1 浓度范围内线性关系良好 ,平均回收率分别为10 0 0 3% ,10 0 2 3%和 99 96 %。样品测定结果与部颁标准方法一致 (P >0 0 5 )。本法具有测定波长少、计算简单、光谱“分离”能力强以及能在低档分光光度计上实现 ,易于推广等特点。

The contents of three components in compound acetylsalicylic acid tablets were determined by dual-wavelength ratio spectrometry. According to the feature of the spectra, 213, 227, 245 and 265 nm were chosen as the determining wavelengths. It is shown that for the three components good linear correlations exist for acetylsalicylic acid (5-20 mug (.) mL(-1)), phenacetin (2-10 mug mL(-1)), and caffeine (2-20 mug (.) mL(-1)). The average recoveries were 100.03%, 100.23% and 99.96% respectively. The results were consistent with those obtained by the standard method of Health Ministry(P > 0.05). The method is simple and practical with fewer determination wavelengths and powerful spectral resolution, and can be performed on a lower level instrument, as well as be easily spreaded for application.