急性心肌梗死血管生成和bFGF表达

Angiogenesis and the expression of bFGF in acute infarcted myocardium

目的 :探讨心肌缺血后血管生成和碱性成纤维生长因子 (basic fibroblast growth factor,b FGF)表达的关系。方法 :2 4只犬建立急性心肌梗死 (myocardial infarction,MI)模型后随机分成对照组 (MI区注射生理盐水 15 m l)和实验组 (MI区注射 b FGF5 0 mg与生理盐水的混合液 15 ml)。每组观察 4个不同时间点 (术后第 1、3、10和 17天 ) ,每个小组 3只犬。免疫组织化学方法检测各组心肌细胞中 b FGF的表达及微血管数量。结果 :除第 1天外各个时间点的微血管数量实验组比对照组显著增多 (对照组和实验组分别为 :第 3天 :92± 12、14 7± 12 ;第 10天 :12 5± 12、183± 14 ;第 17天 :12 5± 15、2 2 4± 2 0 ;单位 :个 /视野 ) ;心肌缺血区治疗组 b FGF的表达增多 (对照组和实验组分别为 :第 1天 :15± 2、35± 7;第 3天 :34± 4、6 4± 6 ;第 10天 :5 5± 9、79± 4 ;第 17天 :2 1± 5、37± 13)。结论 :局部心肌内注射 b FGF有促进 MI区域毛细血管形成的作用 ;心肌缺血区 b FGF的表达与 MI区域毛细血管的密度密切相关。

AIM: To evaluate the correlation between angiogenesis and the expression of bFGF in infarcted myocardium. METHODS: Twenty-four mongrel dogs were randomized into control group and the experimental group. After acute myocardial infarction was made,a mixfure of 50 mg of bFGF and 15 ml of saline was injected into the infarcted myocardium and its border zone in the experimental group, whereas 15 ml saline alone was given to the control dogs. Every 3 dogs in each group was studied on the 1st,the 3rd, the 10th and the 17th days after the operation respectively. Angiogenesis and the expressions of bFGF of the two grups were evaluated by immunohistochemical studies with Ⅷ factor and the antibody of and bFGF. RESULTS:Microvessel density was higher in the experimental group than in the control group excepton the first day (the control group and the experimental group,respectively: the 3rd day, 92±12 and 147±1;the 10th day, 125±12 and 183±14;the 17th day, 125±15 and 224±20). bFGF expressed more in experimental group the control group and the experimental group,respectively: the 1st day, 15±2 and 35±7;the 3rd day, 34±4 and 64±6;the 10rd day, 55±9 and 79±4;the 17rd day, 21±5 and 37±13). CONCLUSION: Intramyocardial administration of basic fibroblast growth factor helps to promote the growth of microvessels in acute myocardial infarction. There is a close relationship between angiogenesis and expression of bFGF in infracted myocardium.