流动注射化学发光法测定阿莫西林的研究

Chemiluminescence Determination of Amoxicillin by Flow Injection Analysis

目的:建立快速测定痕量阿莫西林的新方法。方法:实验发现,在盐酸介质中,高锰酸钾能氧化阿莫西林产生较强的化学发光,据此,采用流动注射技术,建立了一种测定痕量阿莫西林的化学发光分析法。结果:该法的线性范围为1.0×1017 -5.0×10-5g·mL-1;检出限为6.0×10-8 g·mL-1;对1.0×10-5g·mL-1的阿莫西林进行连续11次平行测定,相对标准偏差为1.56%。结论:本方法快捷、简便且具有很高的灵敏度,可用于阿莫西林胶囊中阿莫西林含量的测定,并与药典规定的方法进行对照,结果满意。

Objective: A new flow injection chemiluminescence method for the determination of amoxicillin was described. Method: The reaction between amoxicillin and potassium permanganate in the hydrochloric acid medium gives intensive chemiluminescence. Based on this phenomenon, a new analytical method for the determination of amoxicillin was developed. Results: The linear relationship between the intensity of chemiluminescence and the concentration of amoxicillin was in the range ofl.×10-7-5.0×10-5g·mL -1 with detection limit of 6. 0×10-8 g ·mL-1,The RSD for measurement of 1.0× 10-5g·mL-1 amoxicillin (n = 11) was 1.56%. Conclusion: The proposed method is easy, rapid and sensitive. The proposed method was applied to the determination of amoxicillin in capsule with satisfactory results.