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检测尿脱落细胞中微卫星不稳定性的临床意义 被引量：2

Clinical Implications of Microsatellite Instability in Urinary Exfoliative Cell of the Patients with Bladder Carcinoma
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摘要 目的:探讨膀胱癌患者尿脱落细胞微卫星不稳定性及临床意义。方法:将42例患者尿液标本经适当处理后,采用PCR-PAGE-银染方法检测尿脱落细胞中微卫星不稳定性的情况,分析其与临床病理参数的关系。结果:膀胱癌患者尿中MSI阳性率为59.52%(25/42),浸润性肿瘤患者尿中阳性率较高,但无统计学意义(P>0.05);复发病例中阳性率(80.00%)比初发者(40.91%)明显要高(P<0.05)。结论:检测尿脱落细胞微卫星不稳定性可以作为膀胱癌的一种无创诊断及筛查手段。 Objective: To investigate microsatellite instability (MSI) and it's potential clinical implications in urinary exfoliative cell of the patients with bladder carcinoma. Methods: After treated with proper method, urine spacimens from 69 individuals were analyzed by PCR for microsatellite instability and it's relation to the clinico-pathologic parameters. Results: The overall sensitivity of microsatellite instability in voided urine samples for the detection of bladder neoplasms was 59.52%(25/42). It was relatively higher in the tumor of advanced stage and grade, but there was no significance(P>0.05). It was significantly higher in recurrent cases(60.00%, 12/20) than in primary cases(80.00%, 16/20)(P<0.05). Conclusion: MSI is a potential biomarker for noninvasive detection of bladder carcinoma by assaying exfoliated cells from the voided urine specimen with PCR.
作者 狄金明 吴穷 章尧 李庆文
机构地区 蚌埠医学院附属医院泌尿外科 蚌埠医学院附属医院肿瘤内科 蚌埠医学院检验系
出处 《中国肿瘤临床》 CAS CSCD 北大核心 2005年第2期73-75,78,共4页 Chinese Journal of Clinical Oncology
基金 2000年安徽省教育厅高校优秀青年教师科研基金(编号:2000jq137) 安徽省自然科学基金资助(编号:2001kj176)
关键词 膀胱癌 微卫星不稳定性 尿脱落细胞 Bladder neoplasms Gene Microsatellite instability Urinary exfoliative cell
分类号 R737.14 [医药卫生—肿瘤]
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共引文献37

同被引文献17

  • 1马鹏程,张石生,万江厚,白进良,朱俊芳,赵丽.端粒酶活性检测在膀胱肿瘤早期诊断中的应用[J].中国肿瘤临床,2006,33(2):71-73. 被引量：4
  • 2赵泽驹,罗旭,付逆,罗光恒,郭建桥.尿微卫星不稳定性研究对膀胱肿瘤诊断的探讨[J].临床泌尿外科杂志,2006,21(6):418-420. 被引量：1
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引证文献2

二级引证文献5

中国肿瘤临床
2005年 第2期

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