迟缓液化精液的两种体外处理方法的比较研究

A comparative study on two methods of handling nonliquefaction semen in vitro

目的 比较加糜蛋白酶后上游法和梯度离心法处理液化延长精液的精子质量。 方法 30例正常精液和 34例液化延长精液均取自本室门诊精液常规检测者 ,所有标本量均≥ 2 ml。常规检测后均分为两份 ,一份采用加糜蛋白酶后上游法处理 ,另一份用 Percoll梯度离心法处理 ,显微镜下观察精子的活动力、活动率及 2 4小时存活率。 结果 除不液化伴高粘度的精液外 ,两种方法处理后镜下观测的精子参数经统计学处理差异无显著性。 结论 对液化延长精液采用加糜蛋白酶后上游法方便、有效。

Objective To compare the parameters of nonliquefaction semen handled by the methods of swim up added chymotrypsin and gradient centrifugal. Methods 30 normal semen and 41 nonliquefaction semen were taken from our outpatient department and all the sample's volume was ≥2 ml.After rountine tests, each sample was divided into two parts and its volume was 1 ml respectively. One was handled by swim up added chymotrypsin, the other by gradient centrifugal. Then, by means of microscope, the viability、the rate of mobility and survival for 24 hours at 37℃ in 5% CO2 incubator were observed and compared. Results There was no significant difference between these two methods for the observed parameters. Conclusion It is convenient and effective to handle nonliquefaction semen by the method of swim up added chymotrypsin.