用染色体原位杂交技术定位猪βHCG基因同类物于14q^(11)-q^(16)

LOCALIZATION OF THE HOMOLOGUE TO βHCG GENE ON PIG CHROMOSOME 14q^(11)-q^(16) BY IN SITU HYBRIDIZATION

本研究以点渍法和原位杂交技术为主要方法,以人类绒毛膜促性腺激素(Human Chorionic Go-nadotropin)β链的克隆基因为探针,开展猪的基因定位研究。证明猪的基因组中存在βHCG基因同类物,并将其定位于染色体14q^(11)-q^(16).本文还对一些相关问题进行了讨论。

To map the gene of pigs, dot blotting and in situ hybridization are conducted, using the β HCG as the probe. It is shown that there is the homologue to β HCG gene in the genome of pigs, and it has been assigned to chromosome 14q11-q16. The relevent points of the studies are discussed.