摘要
用荧光标记的鬼笔碱染色,对离体的黄蝉和姜花的生殖细胞内肌动蛋白微丝的分布进行了研究,结果证明两种植物的生殖细胞内部都存在一个微丝网络,黄蝉生殖细胞的比姜花的简单,微丝束较粗。但姜花生殖细胞的网络微丝束比黄蝉的更紧密地环绕着核。用免疫荧光技术在黄蝉生殖细胞的分裂前期和中期,可以观察到一些微丝束的存在,但在分裂后期和末期细胞内的肌动蛋白则变为颗粒状。
Actin filaments were localized in the isolated generative cells of Allamanda schoteii and Hedyehir-um coranarium using fluorescence labeled phalloidin. In the generative cells of A. schuteii an actin cytoskeleton was revealed. This cytoskeleton was characterized by having a network consisting of thick bundles in the cytoplasm and around the nucleus. The pattern of organization of the actin filament cytoskeleton in the generative cell of H. coranarium was slightly different. It possessed a more well developed cytoskeleton consisting of an actin network and a more tightly packed mass of actin filaments around the nucleus. The pattern of distribution of the actin filaments in the dividing generative cells of A. schcteii was also followed. This was done by using immunofluo-rescence technique instead of fluorescence labeled phalloidin; because the fluorescence labeled phalloidin failed to stain the actin in the dividing cells. Using the immunofluorescence technique an actin network in the prophase cell was observed. When the cell entered metaphase the actin filaments reorganized into a spindle-shaped structure. In the anaphase and telophase cell, filaments were no longer seen. The cell became filled with punctate anti-actin fluorescence not unlike that seen in the root-tip cells of wheat by McCurdy and Gunning.
