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酶联免疫吸附法直接测定血清雌二醇 被引量:22

Determination of Estradiol in Serum by Enzyme-linked Immunosorbent Assay
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摘要 本文合成了3种不同载体蛋白的雌二醇抗原,研究了载体蛋白免疫原性对雌二醇抗体产生的影响。应用酶联免疫吸附分析法测定了血清中雌二醇,浓度范围为5~160ng/ml(1.8×10^(-8)~5.9×10^(-7)mol/L).RSD%小于10.0%,检测限为1.97 ng/ml,相当于98.5pg/孔(按空白平均值的二倍标准偏差计算)。另外,本文还建立了一种用考马斯亮蓝G-250测定半抗原与蛋白结合比的方法,提出了一种新的亲和层析法纯化雌二醇抗血清的方法。 We synthesized estradiol antigens with three different carrier proteins, studied the influence of carrier protein's immunogenicity to produce estradiol antibody. Estradiol in serum was determined by the enzyme-linked immunosorbent assay, the linear range of concentration was 5-160 ng/ml, or 1.8×10^(-8)-5.9×10^(-7) mol/L with the relative standard deviation of less 10%. The detection limit was 1. 97 ng/ml, equivalent to 98. 5 pg per well (2 × standard deviation of mean value from zero standard). In addition, we established a new method to estimate the ratio of hapten to carrier protein with coomassic brilliant blue G-250 and purity estradiol antiserum by the affinity chromatography.
出处 《分析化学》 SCIE EI CAS CSCD 北大核心 1994年第2期115-120,共6页 Chinese Journal of Analytical Chemistry
基金 国家教委重点基金资助课题
关键词 雌二醇 酶联免疫吸附 层析法 血清 Estradiol, Enzyme-linked immunosorbent assay, Affinity chromatography
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