我国森林土壤中苏云金芽孢杆菌生态分布的研究

STUDIES ON THE ECOLOGICAL DISTRIBUTION OF BACILLUS THURINGIENSIS IN FOREST SOILS OF CHINA

从我国8个森林立地带(寒温带、中温带、暖温带、北亚热带、中亚热带、南亚热带、高原亚热带、热带)所属的13个自然保护区,采集了0—5cm土层林下土壤样品384个.测定了土壤pH、水分和养分.从中分离观察芽孢杆菌菌落1873个,分离出苏云金芽孢杆菌79株,并对其所属亚种进行了初步鉴定.其平均出土率和分离率分别为14.32%和4.21%.研究了芽孢杆菌和苏云金芽孢杆菌在森林土壤中生态分布的规律及苏云金芽孢杆菌对6种昆虫的室内毒力测定,从中筛选出不少的高效菌株.为研究苏云金芽孢杆菌在我国森林生态系中资源的保护、开发和利用,具有重要意义.

A total of 384 forest soils samples at 0-5cm depth collected from 8 forest site zones (13 Natural Reserves) in China were examined for the distribution of Bacillus and Bacillus thuringiensis. The 79 cultures (4.21%) in 1873 isolates of Bacillus collected referable to the Bacillus thuringiensis. Soil pH, moisture content and soil nutrient were determined. Rules of the ecological distribution of Bacillus thuringiensis of 8 forest site zones in China were analysed. Toxicities against 6 species of insect were tested. Strain some efficient serains were obtained. It was shown that resource of Bacillus thuringiensis is much fruitful in forest soils of China.