流行性出血热患者对野、家鼠型病毒多肽抗原的抗体应答

Antibody Response to Antigenic Polypeplides of Two Viruses Causing Epidemic Hemorrhagic Fever

应用兔抗流行性出血热(EHF)病毒血清及EHF病人血清对野、家鼠型EHF病毒多肽抗原进行免疫识别,发现两型病毒多肽抗原间稍有差异。不同流行地区EHF病人血清大多数可与两型病毒49.6ku(50kd)多肽反应,提示该多肽可能是EHF病毒引起机体抗体应答反应的主要抗原成分。并对EHF病人血清中针对两型病毒多肽抗原的抗体检出率与临床病程及病型的关系进行了观察。

The antigenic polypeptides of two epidemic hcmorrhagic fever (EHF) viruses isolated from A. agrarius and R. norvegicus were compared using immunob-lotting technique. The results here indicated that there was slight difference between the apparent molecular weight of the antigenic polypeptides of these two viruses detected with rabbit sera and sera of EHF pateints. It was found that the 50kd antigenic polypeptide of two viruses could be detected with sera of EHF pateints collected from different areas in China such as Hubei, Anhwei, Henan, Jilin and Shanxi provines. This result suggested that the 50 kd antigenic polypeptide might be the main antigenic component of EHF viruses, stimulating body humoral immu-nological response. In addition, the relationship between EHF. viral polypeptides specific IgG antibody positive rate detected with immunoblotting and clinical manifestations of EHF pateints was also discussed.