Sysmex XE-2100全自动血液分析仪分析有核红细胞的临床应用评价(英文)

Evaluation on the Identification and Counting of Nucleated Red Blood Cells by Sysmex XE-2100 Hematology Analyzer

目的探讨SysmexXE-2100全自动血液分析仪有核红细胞计数的准确性并作评价。方法准确性测定:分别用仪器法和显微镜目测法计数38例抗凝静脉血的有核红细胞数(NRBC%),并将两者的结果进行比较。精密度测定:选择3份有核红细胞数值不同的抗凝静脉血分别用仪器法和目测法测定,每份标本重复计数10次,各自计算出CV值进行比较。结果38份抗凝静脉血仪器法计数的有核红细胞结果与显微镜目测法计数结果之间无显著性差异,经t检验,P>0.05,相关系数r为0.9893,P<0.01。仪器法和显微镜目测法对3份有核红细胞数值不同的标本重复计数的平均CV值分别为7.9%和15.1%,显示出仪器法的精密度明显高于目测法。结论SysmexXE-2100全自动血液分析仪计数有核红细胞是一种较准确、快速、精密的方法,可满足临床诊断、治疗及疗效观察的需要。

Objective To evaluate the identification and counting effeciency of nucleated red blood cells by Sysmex XE 2100 Hematology Analyzer. Methods Accurancy: nucleated red blood cells were counted from 38 specimens by Sysmex XE 2100 Hematology Analyzer and compared with the number counted under microscopy. Precision: 3 specimens with different values were counted for the nucleated red blood cells 10 times by Sysmex XE 2100 Hematology Analyzer and compared with the number counted under microscopy. The CV(%) value was estimated.Results There was insignificant difference between the results obtained from Sysmex XE 2100 Hematology Analyzer and those under microscopy. In the T test, P >0.05,r=0 9893(P< 0 01).CV(%) were 8.1%and 15.8%. It means that the Sysmex XE 2100 is more precise in analyzing the nucleated red blood cells than that under microscopy. Conclusion The nucleated red blood cells count by Sysmex XE 2100 is accurate and fast to obtain clinical data.