摘要
Aim An HPLC method for analyzing eleutheroside B (ELU B) and eleutheroside E(ELU E) , two of the main active substances of Acanthopanax preparations were studied. Methods Thesamples were analyzed on a kromasil ODS column with water-acetonitrile as a gradient mobile phase.The flow rate was 0.8 mL·min^(-1) and detecting wavelengths were 206 nm for ELU B, 220 nm for ELUE, solid phase extraction (SPE) and internal standard-salicin were selected. Results The recoveriesof Acanthopanax tablets and injection were 90.4% - 96.8% and 96.4% - 99.8% for ELU B, 87.7% -93.3%and 95.7% - 98.5% for ELU E, respectively. The linear ranges were 4.45 - 22.25 μg· mL^(-1) (r =0.999 8) and 5.11 - 25.55 μg·mL^(-1) ( r = 0.999 7) respectively. Conclusion This method can savethe time for cleaning the chromatographic system and improve sensitivity for Acanthopanaxpreparations , thus providing a way to evaluate the quality of Acanthopanax preparations.
目的 用高效液相色谱法研究刺五加制剂中 2个主要活性成分刺五加苷B、苷E的含量测定方法。方法KromasilODS柱 ,水 乙腈梯度流动相 ,流速 0 . 8mL·min-1,测定波长刺五加苷B 2 0 6nm ,刺五加苷E 2 2 0nm ,水杨酸作为内标 ,选择了固相萃取条件。结果 刺五加片中刺五加苷B、苷E的回收率范围分别是 90 . 4 %～ 96 . 8%和 87. 7%～ 93 .3% ;刺五加注射液中刺五加苷B、苷E的回收率范围分别是 96 .4 %～ 99. 8%和 95 . 7%～ 98. 5 %。线性范围分别是 4 .4 5～ 2 2 . 2 5 μg·mL-1(r=0 . 9998)和 5. 11～ 2 5 . 5 5 μg·mL-1(r=0 . 9997)。结论 该方法节约了清洗色谱系统的时间 ,提高了测定的灵敏度 ,提供了评价刺五加制剂质量的方法。
