无患子组织培养研究

Study on tissue culture for soapberry

采用无患子SapindusmukorossiGaerth成年结果母树上的越冬芽、半木质化春、夏梢嫩茎芽、叶切块为外植体,在含BA、NAA、2, 4-D、IBA、KT不同浓度及其配比的MS培养基上进行芽的诱导。结果表明,剥除鳞片的越冬芽是无患子快速繁殖最理想的外植体。无患子越冬芽萌发的适宜培养基为:MS+BA1 5mg·L-1 +NAA0 2mg·L-1;春、夏梢芽萌发的适宜培养基为:MS+BA4 0mg·L-1 +NAA0 3mg·L-1;春、夏梢芽切块形成愈伤组织的适宜培养基为:MS+ 2, 4-D1 5mg·L-1 +BA0 2mg·L-1;无患子愈伤组织诱导萌芽的适宜培养基为:MS+BA3 0mg·L-1 +NAA0 2mg·L-1 +KT0 2mg·L-1;适宜的幼苗增殖培养基为:MS+BA1 0mg·L-1 +IBA0 02mg·L-1;适宜的小苗生根培养基为: 1 /2MS+NAA0 5mg·L-1 +IBA0 1mg·L-1。

With the surmount-winter buds,delicate caulis bud of tree branch in spring and summer with half lignification and blade leaf that were picked up from adult female tree of soapberry as explants the tender buds were induced on MS medium which contained different concentration and ratio of BA,NAA,2,4-D,IBA and KT.The results indicated that the best explant was the surmount-winter bud whose scales were peeled for rapid propagation.For soapberry,the suitable medium for inducing surmount-winter bud was MS with 1.5mg·L^(-1) BA and 0.2mg·L^(-1) NAA.The suitable medium for inducing bud of tree branch in spring and summer was MS with 4.0mg·L^(-1) BA and 0.3mg·L^(-1) NA,The suitable medium for inducing formation of callus of blade for spring bud and summer bud was MS with 1.5mg·L^(-1) 2,4-D and 0.2mg·L^(-1) BA.The suitable medium for inducing callus germination was MS with 3.0mg·L^(-1) BA, 0.2mg·L^(-1) NAA and 0.2mg·L^(-1) KT.The suitable medium for proliferation was MS with 1.0mg·L^(-1) BA and 0.02mg·L^(-1) IBA,and the suitable medium for rootage was 1/2 MS with 0.5mg·L^(-1) NAA and 0.1mg·L^(-1) IBA.