期刊文献+

hKv4.3最小功能启动子区与上游抑制元件分析 被引量:1

Analysis of the Minimal Functional Promoter Region for hKv4.3 and Upstream Suppressors
下载PDF
导出
摘要 通过克隆hKv4.3的启动子区和相关的上游调控元件,对hKv4.3基因在转录水平的调控进行了分析.对启动子区5′端一系列的删除突变分析证明,hKv4.3基因的最小功能启动子区是位于转录起始位点附近的-156~+2bp序列.经序列分析发现,这个启动子缺乏典型的TATA-box,却存在另外3个元件,即E-box(CANNTG),CArG-box[CC(A/T)6GG]和CACC-box(GGTGC),其中CArG-box对该启动子活性起关键作用.同时在启动子区找到一个未见报道的大小为10bp的抑制子T,删除抑制子T,则启动子活性增加1倍以上. Activation(depolarization) and recovery(repolarization) of the heart is the result of the orchestrated activity of a variety of ion channels and transporters. Potassium currents such as the transient outward potassium current, I_(to), contribute significantly to phase 1 of repolarization. hKv4.3 is the predominant gene that is believed to underlie I_(to) in the human ventricle. The regulation of hKv4.3 at the transcriptional level was examined by cloning its promoter region and associated negative regulatory elements. Serial deletions of the 5′ flanking region identified the minimal promoter of hKv4.3 at -156 to +2 bp surrounding the transcriptional start site. The promoter is not cell type specific and lacks a canonical TATA box. Three motifs found within the promoter include an E-box(CANNTG), a CArG-box[CC(A/T)_6GG], and a CACC-box. The CArG-box is necessary for transcriptional activity. A novel repressor element named T was identified. The deletion of the T element will at least double promoter activity.
出处 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2005年第5期846-851,F006,共7页 Chemical Journal of Chinese Universities
基金 国家自然科学基金(批准号:30170215)资助.
关键词 hKv4.3 最小功能启动子区 抑制子 hKv4.3 Minimal promoter Repressor
  • 引文网络
  • 相关文献

参考文献10

  • 1Shih H. T.. Tex Heart Inst. J.[J]. 1994, 21(1): 30-41.
  • 2Kong W., Po S., Yamagishi T. et al.. Am. J. Physiol.[J]. 1998, 275(Heart Circ. Physiol. 44): 1963-1970.
  • 3Mansourati J., Le Grand.. Am. J. Physiol.[J]. 1993, 265(4 Pt 2): H1466-1470.
  • 4Wickenden A. D., Kaprielian R., Parker T. G. et al.. J. Physiol.[J]. 1997, 504(2): 271-286.
  • 5Kaab S., Dixon J., Duc J. et al.. Circulation[J]. 1998, 98: 1383-1393.
  • 6Juang G., Kong W., Tomaselli G. F. et al.. Circulation[J]. 1999, 100(18): I424.
  • 7Rose J., Kong W., Tomaselli G. F. et al.. Circulation[J]. 1999, 100(18): I425.
  • 8Po S., Kong W., Tomaselli G. F. et al.. Circulation[J]. 1998, 98: 1-126.
  • 9Kong W., Juang G., Tomaselli G. F. et al.. Circulation[J]. 1999, 100(18): I422.
  • 10Gan L., Hahn S. J., Kaczmarek L. K.. J. Neurochem.[J]. 1999, 73(4): 1350-1362.

同被引文献2

  • 1Kong, W., S. Po, T. Yamagishi, M. D. Ashen, G. Stetten, and G. F. Tomaselli. Isolation and characterization of the human gene encoding the I10: further diversity by ahemative mRNA splicing[J]. Am. J. Physiol., 1998.275 (Heart Circ. Physiol.44) : H1963 - H1970.
  • 2Gan L, Hahn SJ,Kaczmarek LK. CeU type-specific expression of the Kv3.1 gene is mediated by a negative element in the 5'untranslated region of the Kv3.1 promoter[J] .J Neurochem. 1999,73(4) : 1350-62.

引证文献1

;
使用帮助 返回顶部