人卵巢癌裸鼠移植瘤模型的建立及其生物学特性的实验应用研究

Establishment of Transplantable Models of Human Ovarian Carcinoma in Nude Mice and Their Biological Characteristics

研究用１例人卵巢癌淋巴转移的癌组织直接移植于裸鼠皮下，建成一株人卵巢癌移植瘤动物模型，已传至５经２６代。移植成功率达１００％，平均裸带瘤存活中位数为１０２。肿瘤倍增时间为７．１７天。组织学和超微结构形态证实保持了原人肿瘤的特征，有淋巴结转移行为。人类肿瘤染色体特征。保留了分泌癌胚抗原的能力。具有Ｐ５３癌基因蛋白的异常表达。移植瘤细胞可在体外培养并传至５例。流式细胞仪及显微光光度计检测移植瘤。

Tumor tissue of lymph node metastasis of human ovarian carcinoma was directly implanted into nude mice subcutaneously and 26 generations have been propagated successfully. The tumor doubling time was 7. 17 days. Histological and electron microscopic observation identified the same characteristics as those of the human ovarian carcinoma on chromsome pattern,CEA secretion and PS carcino-genesis studying. DNA content of the implanted tumor was measured by flow cy-tometer and microspectrophotometry. The DNA contents of the successive generations were similar to the first generation(DI 1. 05-1. 40). Using transplant tumor tissue,colony antibody to human ovarian carcinoma was made.