Sysmex XE-2100检测网织红细胞计数评价

Evaluation of sysmex XE-2100 in counting reticulocytes

目的对SysmexXE-2100型全自动血细胞分析仪定量检测网织红细胞(RC)的性能进行评价。方法采用SysmexXE-2100定量检测RC,并与手工法进行比较。结果批内平均变异系数(CV)3.69%,天间CV4.36%,携带污染率为零;RC绝对值在(4.0￣770.0)×109/L范围内线性良好(r=0.998);采用SysmexXE-2100检测78例标本的RC值与手工法呈显著正相关(r=0.977,P<0.001)且结果差异无显著性(P>0.05)。结论SysmexXE-2100检测RC准确性好、精密度高,操作简便快速,并能提供较多的RC相关参数,可广泛应用于临床。

[Objective]To evaluate the effect of automated Reticulocyte counting by Sysmex XE-2100. The blood samples of patients and normal subjects were detected by XE-2100 and Miller's method recommended by International Committee for standardization in Haematology(ICSH). The linearity of Sysmex XE-2100 was perfect when RC counting was (4.0~770.0)×109/L (r =0.998), with intra-assay CV 3.69%and inter-assay CV 4.36%, carryover rate of 0%; There were highly correlation between Sysmex XE-2100 and Miller's method in 78 results of patients and normal subjects (r =0.977, P <0.001), and the results were no significant differences (P >0.05). [Conclusion] The RC counting by Sysmex XE-2100 is a highly accurate, precision and easily operated method, it also can provide many new parameters for clinical usage.