鸡堫菌菌丝体液体培养的研究

Study on Liquid Culturing for Mycelium of Termitomyces albuminosus

适合鸡菌菌丝体液体培养的培养基成分是：淀粉１０ｇ，蔗糖２ｇ，蛋白胨２ｇ，ＫＨ＿２ＰＯ＿４０．３ｇ，ＭｇＳＯ＿４·７Ｈ＿２Ｏ０．１５ｇ；最佳Ｃ／Ｎ为３０～４０：１；最适培养温度２６℃；ｐＨ６．４；振荡频率１４０～１４５ｒ／ｍｉｎ，继代培养周期８天．液体培养的鸡菌菌丝体氨基酸总量可达２７３ｍｇ／ｇ干重，其中４２．４１％为人体必需的氨基酸（未含色氨酸）．

The optirnal medium and culture conditions for liquid culturing mycelium ofTermitomyces albuminosus was investigated, It was found that the medium contained 2gsucrose,0.3g KH_2PO_4, 0.15g MgSO_4· 7H)2O, 2g peptone and 10g starch was good forculturing Termitomyces albuminosus to generate the mvcelium on shaker at 140～145r/min. The optimal temperature for the culture was 26C. The C/N of the medium was 30～40:1 and pH was ajusted to 6.4 before autoclaving.The mycelium can be harverstedafter liquid cuIture for 8 days.The total amount of amino acid in such as mycelium was273.9mg/g(dry weight of the mycelium)and 42.41 percentage of which was theessential amino acids(tryptophan was not determinated).