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金黄色葡萄球菌GST-mTSST-1的免疫活性检测方法的研究 被引量：2

Detection for Immune Activity of Glutathione S-transferase and Mutant
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摘要 通过对影响琼脂扩散反应的多种条件进行优化,建立了检测金黄色葡萄球菌无毒诱变中毒性休克综合征毒素1融合蛋白GST-mTSST-1免疫活性的双向免疫琼脂扩散检测方法.检测的灵敏度可达到120ng/mL.此方法具有特异性强、灵敏度高、操作简单等特点,对具有免疫活性的抗原蛋白的定量检测有一定的参考价值. A detection method of immunological activity of S.arueus glutathione S-transferase and mutant toxic shock syndrome toxin 1 fution protein is established by optimizing several conditions for agar-gel double immunodiffusion assay. The sensitivity of the method is 120ng/ml. The method proves simple, specific and sensitive, which is useful for quantitative measurement of immunocompetent GST-mTSST-1 antigen protein.
作者 崔京春 郭海勇 孙传丽 屈嫄 于鑫 常颖 范圣第
机构地区 大连民族学院生命科学学院 吉林农业大学动物科学技术学院
出处 《大连民族学院学报》 CAS 2005年第3期51-53,共3页 Journal of Dalian Nationalities University
关键词 金黄色葡萄球菌 GST-mTSST-1 免疫活性 检测 stapylococcus arueus glutathione S-transferase and mutant toxic shock syndrome toxin 1 immune activity detection
分类号 S852.43 [农业科学—基础兽医学]
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参考文献1

  • 1Dong-Liang Hu, Katsuhiko Omoe, Sanae Sasaki, et al. Vaccination with Non-Toxic Mutant TSST-1 Protects Staphylococcus aureus Infection[J]. Journal of Infectious Diseases, 2003, 188:743-751.

同被引文献20

  • 1FITZGERALD J R, MONDAY S R, FOSTER T J, et al. Characterization of a putative pathogenicity island from bovine Staphylococcus aureus encoding multiple superantigens [ J]. J Bacterio1,2001,183 ( 1 ) :53-70.
  • 2HOLBROOK T C, MUNDAY J S, BROWN C A,et al. Toxic shock syndrome in a horse with Staphylococcus aureus pneumonia[ J ]. J Am Vet Med Assoc ,2003,222 (5):620- 623,601-602.
  • 3HU D L, OMOE K, INOUE F, et al. Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates [ J ]. J Med Microbio1,2008,57 ( 9 ) : 1106-1112.
  • 4SCHLIEVERT P M, STRANDBERG K L, LIN Y C,et al. Secreted virulence factor comparison between methicillin- resistant and methicillin-sensitive Staphylococcus aureus, and its relevance to atopic dermatitis [ J ]. J Allergy Clin lmmunol,2010,125 ( 1 ) :39-49.
  • 5JARRAUD S, MOUGEI C, THIOULOUSE J, et al. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups ( alleles ) , and human disease [ J ]. Infect Immun, 2002,70 ( 2 ) :631-641.
  • 6JOHN C C, NIERMANN M, SHARON B, et al. Staphylococcal toxic shock syndrome erythroderma is associated with superantigenieity and hypersensitivity [ J ]. Clin Infect Dis ,2009,49( 12 ) : 1893-1896.
  • 7CUI Jing-chun, ZHANG Bao-jun, LIN Yang-chun, et al. Protective effect of glutathione S-transferase-fused mutant staphylococcal enterotoxin C against Staphylococcus aureus- induced bovine mastitis [ J ]. Vet Immunol Immunopathol, 2010,135 (1-2) :64-70.
  • 8KUROISHI T, KOMIN K, KAI K, et al. Concent rations and specific antibodies to staphylococcal enterotoxin C and toxic shock syndrome toxic 1 in bovine mammary gland secretions, and inflammatory response to the intramammary inoculation of these toxins [ J ]. Vet Med Sci,2003,65 ( 8 ) :899-906.
  • 9WOODY M A, KRAKAUER T, STILES B G. Staphylococcal enterotoxin B mutants (N23K and F44S) : Biological effects and vaccine potential in a mouse model[ J]. J Vaccine, 1997,15 : 133-139.
  • 10HU D L, KATSUHIKO O, SANAE S, et al. Vaccination with non-toxic mutant TSST-1 protects Staphylococcus aureus infection [ J ]. Journal of Infectious Diseases, 2003,188 (9) :743-751.

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大连民族学院学报
2005年 第3期

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