西部地区主要猪种和野猪H-FABP基因分子标记与IMF含量关系

Relationship Between Molecular Marker of Western Main Pig H-FABP Gene and IMF Content

利用PCR RFLP(HinfⅠ、HaeⅢ和MspⅠ3种限制性内切酶)分子标记技术,检测了杜洛克猪、长白猪、大白猪、内江猪、荣昌猪、汉江黑猪、汉中白猪、八眉猪和野猪共计265头猪H FABP基因5′上游区和第二内含子区的遗传变异,并利用最小二乘模型分析了H FABP基因对猪肌内脂肪含量的遗传效应。结果表明:(1)在HinfⅠRFLP位点上,上述品种和野猪均存在多态性,其中大白猪、八眉猪、汉江黑猪、汉中白猪和野猪表现为低度多态,杜洛克、长白猪、内江猪和荣昌猪为中度多态;除汉江黑猪(P<0.05)和野猪(P<0.01)外,其他猪种基因频率和基因型频率都处于Hardy Weinderg平衡状态(P>0.05);而在HaeⅢRFLP和MspⅠRFLP位点上,仅内江猪、荣昌猪、汉江黑猪和八眉猪为单态;(2)9种基因型对肌内脂肪(IMF)含量的影响,HH>Hh>hh,DD<Dd<dd,AA<Aa<aa,遗传效应值分别为:3.89;3.42;3.17;2.27;2.49;2.91;2.28;2.70;2.95;H FABP基因可显著地提高IMF含量(P<0.05)。结果提示:可通过提高“aa dd HH”基因型的频率来增加IMF含量,达到改善猪肉质的目的。

By using 265 pigs from eight breeds including Duroc,Landrace,Large White,Neijiang,Rongchang,Hanjiang Black,Hanzhong White,Bamei and wild ones, the genetic variations of 5`-upstream region from and the second intron in porcine H-FABP gene were checked by PCR-RFLP molecular marker with HinfⅠ、HaeⅢ and MspⅠ,and effect of H-FABP gene on IMF content was then analyzed by least square analysis.The results showed as follows:(1) 8 pig breeds and wild pig had polymorphism at HinfⅠ-RFLP site. In above detected breeds,large white,Bamei pig, Hanjiang Black,Hanzhong White pig breeds and wild pig presented low polymorphism while other breeds have mediate polymorphism;(2)Among the tested breeds only 4 Chinese local pig breeds had no polymorphism at the HaeⅢ-RFLP and MspⅠ-RFLP sites,but Duroc,Landrace,Largewhite, Hanzhong White pig breeds and wild pig had polymorphism. Wild pig at the HaeⅢ-RFLP, Landrace,Largewhite and wild pig at the HaeⅢ-RFLP and MspⅠ-RFLP sites were low polymorphism,others were mediate polymorphism;(3)H-FABP gene increased IMF content significantly(P<0.05). Genetic effect of H-FABP gene on IMF content were HH>Hh>hh,DD<Dd<dd,AA<Aa<aa and genetic effect values were 3.89,3.42,3.17,2.27,2.49,2.91,2.28,2.70,2.95 respectively. The results suggest that porcine meat quality may be improved by increased genotype aa-dd-HH frequency in porcine group.