摘要
本研究以^(?)s标记的人类绒毛膜促性腺激素(humgn chorionic gonadotropin)β链(βHCG)的克隆基因(cDNA)为探针,分別与人类、草鱼(Cteaopharynogodon idellus)和枯草芽胞杆菌(Bacillus subtilis)的总DNA进行点杂交,以~3H标记的同一探针与草鱼中期染色体作原位杂交,结果表明:草鱼基因组中存在βHCG基因的同类物,这一同类物的基因座位在草鱼m组一染色体(可能是第4号染色体)一个臂的末端。本文对所得结果的意义进行了讨论。
Hybridization of a cloned α-S-labelled cDNA probe homologous to the human chorionic gonadotropin β subunit (3HCG) to the spots of total DNA from liver tissue of grass carp (Ctenopharynogodon idellus) on the nitrocellular filter shows there is the homologue to βHCG gene in fish genome.This gene homologue was assigned to terminal portion on an arm of a m-group chromosome of grass carp by in situ hybridization.The significance of finding and mapping the βHCG gene homologue was discussed.
基金
国家自然科学基金
关键词
草鱼
ΒHCG
原位杂交
基因定位
In situ hybridization,Gene mapping,Human chorionic gonadotropin,Fish genetics
