不同浓度DEET软膏制剂对小鼠日本血吸虫感染防护效果比较

Comparison of effect of DEET with different concentrations in prevention of Schistosoma japonicum infection in mice

目的探讨不同浓度N,N-二乙基-间-甲苯甲酰胺(DEET)对小鼠感染日本血吸虫的防护效果.方法将昆明鼠随机分为4组,其中3组为实验组,分别涂抹10%、20%、30%DEET凡士林软膏;1组为对照组,涂抹不含DEET的凡士林软膏.实验组和对照组小鼠涂药后分别浸泡10 min、0.5、1、2、4 h,然后感染尾蚴.各组小鼠均经腹部感染尾蚴(50±5)条,于感染后6～7周处死小鼠,经心脏灌注法收集成虫,计数后计算减虫率及进行秩和检验.结果涂药浸泡1、2、4 h后感染尾蚴,10%、20%和30%DEET凡士林软膏组小鼠获得的减虫率分别为:78.34%、63.15%、40.23%;98.61%、93.37%、75.74%和100.00%、98.61%、93.07%,经统计学分析,3个实验组小鼠体内虫体计数差异均有显著性(H值分别为10.60、10.84和10.80,P均＜0.01).结论DEET凡士林软膏对小鼠感染日本血吸虫的防护效果随DEET浓度的增加而加强.

Objective To explore the efficiency of N,N-diethyl-m-toluamide （DEET） with different concentrations in prevention of Schistosoma japonicum infection in mice. Methods Kunmingmice were randomly divided into four groups for the application of the vaseline ointment with different DEET concentrations （0, 10%, 20% and 30%）. After the abdominal skin of the mice were smeared with those DEETs above mentioned and dipped into water for 10,30 min, 1,2 h and 4 h,the mice were infected with （50±5） cercariae respectively. After infected for 6-7 weeks, all of the mice were sacrificed,and by perfusing heart the worms were collected and counted. Worm reduction rates were calculated and the rank sum tests were carried out. Results At the time points of 1,2 and 4 h , the worm reduction rates were 78. 34%,63.15% and 40. 23% for 10% DEET vaseline ointment, 98.61%,93. 37% and 75. 74% for 20% DEET vaseline ointment and 100.00% ,98.61%and 93.07% for 30% DEET vaseline oinment respectively（H =10.06,10.84,10.80,all P〈0.01）.Conclusion With the increasing of the concentration of DEET, the efficiency of DEET vaseline ointment is enhanced in preventing Schistosoma japonicum infection in mice.