热锻炼能提高菜豆细胞可溶性蛋白、SOD、膜ATP酶和质子泵在体外的热稳定性

Heat Acclimation Can Increase the Thermotolerance of Soluble Proteins,SOD,ATPase and H￣+ Pump in vitro

以菜豆为实验材料，研究了蛋白质分子和生物膜在体外热稳定性的变化和原因。实验结果表明：热锻炼可增加细胞的耐热性，并赋予ＳＯＤ、膜ＡＴＰ酶和生物膜质子泵在体外的热稳定性；通过对可溶性蛋白热变性后的浊度分析和用ＡＮＳ荧光探针探测蛋白质的结构变化，发现蛋白热变性的原因是疏水区的暴露和分子相互团聚，^（３５）Ｓ－Ｍｅｔ标记分析和二维电泳谱揭示，热锻炼诱导合成的热激蛋白具有阻止蛋白质热变性和生物膜热破坏的功能。

Phaseolus Vulguris was chosen as test material. The purpose of this research was to study the phenomenon and causes of in vitro heat resistance of proteins and biomembranes which were extracted from the bean. Heat acclimation rendered not only increased the bean cell thermotolerance but also in vitro heat stability of SOD,ATPase and membrane vesicles H￣+-pump. Scattering and ANS fluorescence of denatured proteins indicated that exposing of the hydrophobic internal of the protein and futher coalition of molecules made proteins lose activities.￣ (35)S-Met labelling and two-dimention electrophoresis showed that heat shock proteins which were expressed during heat acclimation can slow down the protein heat denaturation and biomembrane destruction. It also showed that more rapid accumulation of low molecular weight and 70 kD heat shock proteins than others might play major role.