CEA高表达人肺腺癌裸鼠移植瘤模型的建立

Establishment of a Model of Transplantation of Human Lung Adenocarcimoma with High Expression of CEA in Nude Mice

将１例人肺高分化腺癌手术标本直接移植于ＮＩＨ裸小鼠皮下，建立了ＣＥＡ高表达人肺高分化腺癌裸小鼠移植瘤模型（ＬＡＸ─９１），裸鼠间已传３２代，历时２年余。ＬＡＸ─９１潜伏期短，生长稳定，移植成功率１００％。各代移植瘤病理组织学观察均保持了原病人肿瘤的结构，并且有分泌功能，染色体分析显示人恶性肿瘤特征。血清ＣＥＡ含量多次测定结果表明，ＬＡＸ─９１具有稳定高分泌ＣＥＡ的生物学特性。该移植瘤模型的建立，为进一步研究人肺癌和癌胚抗原的关系提供了有用的手段。

A model of the transplantation of human lung adenocarcinoma with highexpression of CEA in nude mice(LAX-91)was established by the direct transplantation ofa surgical specimen from a patient with well-differentiated Iung cancer subcutaneously inthe NIH nude mice.The transplanted tumor had been passed on for more than two yearsthrough 32 passages.The LAX-91 tumor had short incubation Periods and grew quitesteadily.The rate of success was 100%. Pathohistological obseryation of the transplantedtumor in each generation showed that it had maintained the structure of the original human tumor and had the function of secretion.Chromosomal analysis revealed that the tumor cells had the characteristics of the human malignant tumor.The results of serum CEA con-tent determination for various times indicated that the LAX-91 tumor had possessed the biological claracteristics of secreting CEA stably and highly. The establishment of such a transplanted tumor model provides a useful method for the further studying of the relations between human Iung cancer and CEA.