Isolation and Identification of a Taxol-producing Endophytic Fungus Identified from Taxus media

一株产紫杉醇的曼地亚红豆杉内生真菌的分离及鉴定(摘要)(英文)

[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.

[目的]分离并鉴定1株产紫杉醇的曼地亚红豆杉内的内生真菌。[方法]从曼地亚红豆杉树皮内表皮中分离得到32株内生真菌,并通过高效液相色谱法检测其发酵产物。[结果]筛选获得1株可以产紫杉醇的内生真菌M57,其紫杉醇产量为45～50μg/L,并通过对M57菌落的形态学观察以及18S rDNA序列分析初步将其鉴定为根霉属(Rhizopus)真菌。[结论]该菌株的发现为微生物发酵法生产紫杉醇提供了具有潜在应用价值新的菌种。