摘要
南美香瓜梨茎段被移植在MS基本培养基上,其中添加有BA6.0(mg/L以下单位相同)、IBA0.2培养4周后增殖到3.8倍。用其嫩叶切块,在含有2,4—D0.5,BA0.25的MS培养基上先诱导形成绿色愈伤组织,然后转入含BA6.0和IBA0.2的MS培养基上,产生丛生不定芽。将2.5~3.0cm的不定芽切下移入含IBA0.5与0.2%活性炭,1/2MS无机盐的培养基上,25天后诱导生根,移栽成活率达90%以上。
The stem cuttings of Solanum muricatum Ait.were inoculated on MS base medium supplied with BA 6.0 mg/L(same unit in following supplemented mecdium),IBA 0.2. After 4 weeks of culture,the multiplication of buds were 3.8 times.The small cuttings of leaf tissue were induced to callus on the MS medium supplied with 2,4-D 0.5 and BA 0.25. Then many buds were induced on the callus by culturing on the MS medium supplied with BA 6.0 and IBA 0.2.The plantlets with many roots were grown on the 1/2 MS medium suppplied with IBA 0.5 and 0.2% active carbon, after 25 days of culture.The plantlets were transplanted into the soil and the rate of survivers were up to 90%.
出处
《广西植物》
CAS
CSCD
北大核心
1996年第1期69-72,共4页
Guihaia
关键词
香瓜梨
离体培养
快速繁殖
草本果类
Solanum muricatum
culture In Vitro
rapid propagation