维生素E琥珀酸酯促进Fas表达和诱导胰腺癌细胞凋亡

The mechanism of vitamin E succinate induced apoptosis of human pancreatic cancer cells: by up-regulation of Fas expression on the surface of cancer cells

目的:检测维生素E琥珀酸酯(VES)对胰腺癌细胞生长抑制和凋亡的诱导作用,并分析其对凋亡诱导分子Fas表达的调控作用。方法:用VES刺激人胰腺癌Bxpc-3细胞12、24或48 h,所用VES浓度分别为5、10或20 mg/L;以MTT法测定其对细胞生长的抑制作用,流式细胞仪分析细胞凋亡率和细胞表面的Fas表达水平,Western印迹法检测VES对Fas蛋白水平的影响。结果:VES对胰腺癌细胞具显著的生长抑制作用,并表现呈剂量与时间依赖关系。用流式细胞仪分析细胞周期的结果,未经VES处理的Bxpc-3细胞的凋亡率为1.3%;5、10及20 mg/L VES作用48 h后,凋亡率分别为10.6%、24.2%和68.7%。VES作用后,胰腺癌细胞Fas蛋白水平和细胞表面的Fas表达升高。结论:VES对胰腺癌细胞具显著的生长抑制作用并诱导胰腺癌细胞凋亡,其机制与促进细胞表面Fas表达的上调有关。

Objective To investigate the growth inhibitory effect of vitamin E sueeinate（VES） on human pancreatic cancer cells and its mechanism. Methods Human pancreatic cancer cells Bxpc-3 were treated with VES for 12h, 24h and 48h, respectively. The concentrations of VES used were 5mg/L, 10mg/L and 20mg/L, respectively. The growth inhibitory effect was measured with MTF and the cell cycle and cell surface Fas expression were analyzed by flow cytometry. Western blot assay was performed to detect Fas protein expression. Results VES exercised a significant growth inhibition and apoptosis induction effect on Bxpe-3 human pancreatic cancer cells. The apoptotic rate rose from 1.3% to 10.6%, 24.2% and 68.7% after administration of VES. Both Fas protein level and cell surface Fas expression increased after being treated with VES. Conclusions VES had significant inhibitorv effect on Bxpe-3 human pancreatic cancer cells. The mechanism is related to Fas up-regulation on the surface of cancer cells.