金属细菌叶绿素的合成及其对肿瘤细胞的抑制作用

Synthesis and antitumour activity of metal complexes of bacteriochlorophyll

目的寻找一种新型光动力学疗法光敏剂。方法采用从光合细菌中分离纯化的脱镁细菌叶绿素为配体,与金属盐在有机溶剂中反应,合成了Cu,Zn,Co,N i 4种金属细菌叶绿素,并对其紫外可见光谱和荧光光谱进行研究。此外还研究了4种金属细菌叶绿素对K562和HL60两种白血病细胞生长的影响。结果4种金属细菌叶绿素的光谱图都有可预见的漂移,证明金属已配位到细菌叶绿素的卟啉大环上。同时,4种金属细菌叶绿素都有很强的抑瘤作用,光照可以明显提高其抑瘤率。结论金属细菌叶绿素作为一种新型光敏剂具有优良的性质,是新一代光敏剂发展的一个方向。

Aim To find a new PDT sensitizer. Methods There were four complexes （Cu, Zn, Co, Ni） synthesized through reaction of metal and deprivating-Mg bacteriochlorophyll in the organic solvent. Their antitumor action was detected by MTT. Results The ultraviolet-visual spectrum and the fluorescence spectrum of these complexes showed that synthesis of these four complexes was succeeded. And these metal complexes have potent antitumor action on two kinds of leukaemic cells. Conclusion Metal bacteriochlorophylls as PDT sensitizers have very good properties and this is a way to develop new PDT sensitizers.