氦氖激光对牙龈卟啉菌杀菌作用及形态影响的实验研究

Experimental Research on the Effect of He-Ne Laser on Porphyromonas Gingivalis

目的:研究氦氖激光照射对牙龈卟啉菌杀菌及形态的影响,以期寻求治疗牙周炎的新手段.方法:用功率为6.3 mW的氦氖激光照射牙龈卟啉菌,将细菌分为1个空白对照组和5个实验组,分别用不同照射时间(16、32、48、64、80 min)的氦氖激光(波长632.8 nm,功率6.3 mW)进行照射,数活菌数和透射电镜下观察其形态学改变.结果:氦氖激光照射前后活细菌数无显著变化,但菌体的形态产生明显固缩.结论:单纯氦氖激光照射Pg,无明显的杀菌作用,但能使菌体形态发生变化.

Objective： To find a new way for curing periodontitis through investigating the influence of He - Ne laser on porphyromonas gingivalis （Pg）. Methods： Porphyromonas gingivalis （Pg） were irradiated by the He- Ne laser which had a power output of 6.3 mW. The bacterium of Pg were separated into 6 groups which including 5 experimental groups and 1 negative control group. The experimental groups were irradiated with He - Ne laser （wavelength： 632.8 nm, power of output： 6.3 mW） at different time （16, 32, 48, 64, 80 min）. Living bacterium of Pg were counted after treated and the morphological changes of Pg were observed by transmission electron microscope. Result： The amount of living bacterium： there is no sig- nificant difference before and after irradiated by the He - Ne laser at different time. Transmission electron microscope： the longer time the bacterium were exposed under the He- Ne laser, the more significant shrinkage there was. Conclusion： There was no significant inhibition or killing effect of He- Ne laser on the bacterium of Pg.