血清中不同基因型丙型肝炎病毒RNA含量的测定

Quantitative analysis of HCV RNA among different genotypes in serum

目的了解血清中不同基因型丙型肝炎病毒HCVRNA的含量。方法用实时荧光定量RTPCR和逆转录型特异性引物PCR同时检测21例费城酒精依赖丙肝患者的血清HCVRNA拷贝数并分析HCV基因型。结果21份血清标本中,有17份为HCVRNA阳性。这些阳性血清的HCVRNA含量波动范围在104.60～106.20拷贝/ml。检测到1a和1b两种HCV基因型,其中1a型7例,RNA平均滴度为105.28±0.75拷贝/ml,1b型(9例)RNA平均滴度为105.28±0.28拷贝/ml。结论定性和定量检测结果有极佳的一致性,酒精依赖丙肝基因型1a和1b血清中HCV含量无显著性差异。

Objective To analyze HCV genotypes and quantify HCV RNA in sera isolated from alcohol abusers in Philadelphia area by real-time RT-PCR. Methods Both real-time fluorescence quantitative RT-PCR and reverse transcription specific primers PCR was applied to analyze serum HCV RNA copy numbers and genotypes. Results Out of 21 serum specimens, 17 were positive for HCV RNA. The copy numbers of HCV RNA ranged from 1 ×10^4.60 copies/mlto 1 × 10^6.20 copies/ml. Two HCV genotypes were identified among 17 positive sera, type la （ 7 cases） and type 1b（9 cases）. The mean titer of HCV RNA in sera from HCV 1a-infected subjects was 10^5.28±0. 75 copies/ml, while that in HCV 1b-infected subjects was 10^5.20± 0.28 copies/ ml. Conclusion Our data showed that to analyze HCV core region is practically feasible for HCV genotyping, and there is no statistical differences in HCV RNA serum levels between HCV 1a-infected subjects and HCV 1b-infected subjects.