核因子-κB对新生儿败血症的早期诊断价值

Value of Nuclear Factor-κB for Early Diagnosis on Neonatal Sepsis

目的探讨核因子κB(NFκB)活化与新生儿败血症的关系。方法将57例感染新生儿分为败血症组(26例)和非败血症组(31例)。正常新生儿20例为对照组。采用流式细胞仪分别检测患儿入院、入院后24及48h外周血单个核细胞(PBMC)中NFκB的表达;患儿均于使用抗生素前作血培养。结果败血症组PBMC中NFκB表达水平明显高于非败血症组和对照组(P均<0.01),而非败血症组与对照组比较无显著意义(P均>0.05);败血症组NFκB入院时明显高于入院后24、48hNFκB(P均<0.0001),24h与48h时NFκB比较无显著差异(P>0.05);非败血症组在入院时、入院后24、48hNFκB的表达水平均无显著差异(P均>0.05)。结论NFκB可作为新生儿败血症早期诊断指标之一,并指导临床治疗。

Objective To investigate the expression and the effect of nuclear factor-κB（NF -κB） in neonatal sepsis. Methods We separated 77 newborn infants into 3 groups, which were septic group （26 cases）,non- septic group （31 eases） and control group （20 cruses）. NF-κB, existed in PBMC was detected in 3 different periods, including at admission, after the 24th hour and 48th hour of admission, of the septic group and the non- septic group by flow cytometry. At the same time, the sample of the septic group and the non - septic group were drawn for blood cultures at admission before using antibiotics. Results The expression of NF -κB in septic group was more significant than that in the other 2 groups （P〈 0.01 ）. And there was no significant difference between the non - septic group and control group. Moreover, the expression of NF -κB, in septic group, increased at admission and decreased after the 24th hour, but it was no difference between the 24th hour and 48th hour. And, the expression of NF-κB was also no significant difference at the 3 different periods in non - septic group. Conclusions It has been demonstrated that NF-κB increased in early period of neonatal sepsis, and not only can be used as a monitor standard in the early diagnosis of neonatal sepsis, but also can direct the therapy. Furthermore, the flow cytometry used in the NF-κB detecting, which considered as a sensitive and convenient method, can easily be used in most hospitals.