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蜕膜细胞条件培养液中TNF-α及EGF对卵巢癌细胞株COC1侵袭基因MMPs/TIMPs表达的影响 被引量：1

Effect of TNF-α and EGF in decidual cell conditioned media on the experssion of cocl invasive gene MMPs/TIMPs in ovarian fumor cell line
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摘要 目的研究蜕膜细胞条件培养液(decidual conditioned media,DCM)中TNF-α及EGF对卵巢癌细胞株COC1侵袭基因基质金属蛋白酶(MMPs)/基质金属蛋白酶抑制剂(TIMPs)表达的影响。方法原代培养早孕蜕膜细胞并提取DCM,用DCM及分别加入TNF-α及EGF抗体的DCM处理COC1,利用RT-PCR法对COC1侵袭基因MMPs/TTMPs的表达进行分析。结果COC1表达MMP-2,TIMP-2,而不表达MMP-9,TIMP-1。早孕EGF-Ab组与对照组(DCM组)比较,前者可以使COC1的MMP-2 mRNA表达降低,且差异有显著性(P<0.01)。而TNF-α抗体组可以降低MMP-2 mRNA的表达,但与对照组比较差异无显著性(P=0.280)。与DCM组比较,EGF-Ab组可以使COC1的TIMP-2 mRNA表达增加,两者差异有显著性(P<0.01)。而TNF-α抗体组有增加TIMP-2 mRNA表达,但差异无显著性(P>0.05)。结论早孕DCM中TNF-α可能抑制COC1侵袭力。早孕DCM中EGF可能促进COC1侵袭力。
作者 朱金虎 陈士岭 邢福祺 庞战军
机构地区 南方医科大学南方医院生殖医学中心
出处 《广东医学》 CAS CSCD 北大核心 2005年第12期1644-1646,共3页 Guangdong Medical Journal
基金 "973"国家重点基础研究发展规划项目(编号:G1999055903)
关键词 蜕膜务件培养液 肿瘤坏死因子-Α 上皮生长因子 COC1细胞株 卵巢癌细胞 侵袭基因
分类号 R737.31 [医药卫生—肿瘤] R446.5 [医药卫生—诊断学]
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参考文献8

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共引文献23

同被引文献8

  • 1朱金虎,庞战军,陈士岭,邢福祺.蜕膜细胞条件培养液对卵巢癌细胞株COCl侵袭相关基因表达的影响[J].第一军医大学学报,2004,24(7):752-755. 被引量：2
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引证文献1

广东医学
2005年 第12期

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