旋毛虫感染小鼠对p46000重组抗原的抗体应答

Antibody Response of Mice Infected with Trichinella spiralis on p46 000 Recombinant Antigen

分别以旋毛虫肌幼虫ES抗原和p46000重组蛋白作为抗原，对小鼠人工感染旋毛虫后的抗体应答进行了ELISA检测。结果表明，以肌幼虫200条／只经口感染小鼠后，肌幼虫ES抗原在感染后9d可检出抗体，并于感染后35～42d达到最高水平；应用重组抗原检测时，感染后10d可检出抗体，抗体水平略低于用ES抗原，但是其消长规律基本一致．而且与阴性血清相比差异明显；抗体在117d后仍维持于较高水平。

p46 000 recombinant antigen of Trichinella spiralis was used in ELISA to detect anti-p46 000 antibody of mice infected with T. spiralis. The results showed that the antibody in mice infected with 200 muscle larvae per mice could be detected by T. spiralis muscle excretory-secretory （ES） antigen on 9 d and reached the peak from 35 to 42 d. When p46 000 recombinant antigen was used, the antibody against p46 000 could be detected on 10 d and maintained at a high level until 117 d. the response regulation of anti-p46 000 antibody was similar to that of anti-ES antibody,