Erythropoietin -induced proliferation of gastric mucosal cells

AIM： To analyze the localization of erythropoietin receptor on gastric specimens and characterize the effects of erythropoietin on the normal gastric epithelial proliferation using a porcine gastric epithelial cell culture model. METHODS： Erythropoietin receptor was detected by RT-PCR, Western blotting and immunohistochermistry. Growth stimulation effects of erythropoietin on cultured gastric mucosal cells were determined by ELISA using bromodeoxyuridine （BrdU）. RESULTS： Erythropoietin receptor was detected on cultured porcine gastric mucosal epithelial cells. Erythropoietin receptor was also detected histochemically at the base of gastric mucosal epithelium. BrdU assay demonstrated a dose-dependent increase in growth potential of cultured porcine gastric mucosal epithelial cells by administration of erythropoietin, as well as these effects were inhibited by administration of antierythropoietin antibody （P〈 0.01）. CONCLUSION： These findings indicate that erythropoietin has a potential to proliferate gastric mucosal epithelium via erythropoietin receptor.

瞄准：在胃的标本上分析红细胞生成素受体的本地化并且用一个猪的胃的上皮的房间文化模型在正常胃的上皮的增长上描绘红细胞生成素的效果。方法：红细胞生成素受体被 RT-PCR，西方的弄污和 immunohistochermistry 检测。有教养的胃的粘膜房间上的红细胞生成素的生长刺激效果被 ELISA 用 bromodeoxyuridine (BrdU ) 决定。结果：红细胞生成素受体在有教养的猪的胃的粘膜上皮细胞上被检测。红细胞生成素受体也在胃的粘膜上皮的底组织化学地被检测。BrdU 试金由红细胞生成素的管理在有教养的猪的胃的粘膜上皮细胞的生长潜力表明了剂量依赖者增加，以及这些效果被反的管理禁止 -- 红细胞生成素抗体(P<0.01 ) 。结论：这些调查结果显示红细胞生成素有一个潜力增殖经由红细胞生成素的胃的粘膜上皮受体。