流动注射化学发光抑制法测定痕量抗坏血酸

Determination of Trace Ascorbic Acid by Flow Injection Chemiluminescence Suppression Method

研究发现在碱性介质下，抗坏血酸对 Luminol-K3Fe（CN）6体系发光反应具有强烈的抑制作用，建立了化学发光抑制快速测定痕量抗坏血酸的新方法。本法测定抗坏血酸的线性范围为4．0×10－8～1．0×10-6mol／L，D．L＝40×10－8mol／L，对1．0×10－7mol／L抗坏血酸连续11次测定的相对标准偏差为0．5％，应用于医用维生素C片剂、维生素针剂以及血样中抗坏血酸的测定，结果满意。

A highly sensitive and rapid chemiluminescence suppression method for the determination of ascorbic acid based on luminol-K3Fe (CN)6-ascorbic acid system is developed.The linear range for ascorbic acid is 4. 0 × 10-8~1.0× 10-6 mol/L with a detection limit of 4.0 × 10-8 mol/L. The relative standard deviation for 1. 0×10-7 mol/L ascorbic acid is 0. 5 %(n= 11). The method gives good results to ascorbic acid determination in vitamin C injection, Vitamin C tablet and blood samples.