Enzymatic Transformation of Notoginsenoside Fe by β-Glucanase

β-葡聚糖苷酶对三七皂苷-Fe的转化研究(英文)

Aim An industrial enzyme β-glucanase was used to transfortn notoginsenoside Fe for the first time. Methods Notoginsenoside Fe was isolated from the leave saponin of Panax notoginseng （Burk.） Chen FH. The enzymatically transformed compounds were detected by HPLC and two transformed compounds were identified as 20 （S） -protopanaxadiol-20- O- α-L-arabinofuranosyl （ 1→6 ） - β-gluco- pyranoside, ginsenoside-Mc） and 20（S）-protopanaxadiol-20-O-β-D-glucopyranoside compound-K （C-K） respectively on the basis of their ^1H NMR and ^13 C NMR spectral data. Results Based on the enzymolytic kinetic curve, the transformation rate of notoginsenoside Fe reached 95% after 24 h. Conclusion The enzymatic transformation pathway of notoginsenoside Fe by β-glucanase has been proposed as notoginsenoside Fe→ginsenoside Mc→C-K.

目的本文首次报道利用工业酶制剂对三七皂苷-Fe进行酶转化的研究。方法从三七茎叶总皂苷中分离鉴定三七皂苷-Fe,利用工业酶制剂β-葡聚糖苷酶对三七皂苷-Fe进行转化。树脂法、硅胶柱层析及薄层层析法分离、纯化酶转化产物;1HNMR和13C NMR光谱数据分析鉴定酶转化产物Ⅰ是20(S)原人参二醇-20-O-α-L-呋喃阿拉伯糖基(1→6)β-D-吡喃葡萄糖苷(人参皂苷-Mc);转化物Ⅱ是20(S)原人参二醇-20-O-β-D-吡喃葡萄糖苷(C-K)。结果酶解动力学的研究中,经过24h的反应,三七皂苷-Fe的转化率接近95%。结论β-葡聚糖苷酶转化三七皂苷-Fe的途径为三七皂苷-Fe→人参皂苷-Mc→C-K。