野生及栽培甘草HPLC指纹图谱

Studies on the HPLC Fingerprint of Radix Glycyrrhizae

目的:研究野生及栽培甘草的质量区别。方法:建立野生及栽培甘草的HPLC指纹图谱(Cro-masil ODS色谱柱;0.1%磷酸溶液为流动相;梯度洗脱)并进行对比分析;利用MS/MS技术推测化合物结构。结果:鉴定了部分成分的化学结构;野生与栽培甘草所含成分大部分相同,仅有少量区别;栽培甘草中少数成分随栽培年限不同有所变化。结论:本方法灵敏度高,分析快速、准确,可以为甘草药材内在质量控制提供依据。

AIM： To study the quality disparities between wild and cultivated licorice root. METHOD： HPLC fingerprint of Radix Glycymhizae was established （Cromasil ODS column; 0.1% phosphoric acid-acetonitrile as mobile phase; gradient elute, etc）; The HPLC fingerprints of wild and cultivated licorice root was compared. RESULTS： Some peaks were identified by MS/MS; The fingerprints of them had lettle difference; The contents of two constituents in cultivated licorice root of different growing years were varied dynamically. CONCLUSION： The method is convenient, accurate and suitable for the quality control of Radix Glycyrrhizae.