VEGF、VEGF-C、Flt-4蛋白和MVD、LVD在乳腺癌组织中的表达及临床意义

Expression of vascular endothelial growth factor (VEGF), vascular endothelial growth factor C(VEGF-C), fms-like tyrosine kinase 4(Flt-4), microvascular desity (MVD) and lymphatic-vessel density (LVD) in breast cancer and its clinical significance

目的探讨乳腺癌组织中VEGF、VEGF-C、Flt-4蛋白表达水平、MVD、Flt-4阳性脉管数及LVD六项指标之间的相互关系及其临床意义。方法采用免疫组织化学S-P法,检测105例乳腺癌石蜡标本中VEGF、VEGF-C、Flt-4、MVD、LVD及Flt-4阳性脉管数的表达水平。结果VEGF表达水平与MVD有显著相关性(P<0.01);VEGF-C表达水平与Flt-4表达水平有显著相关性(P<0.01),且二者均与Flt-4阳性脉管数及LVD有显著相关性(P<0.01)。在淋巴结转移组中,VEGF-C蛋白表达水平、Flt-4阳性脉管数及LVD均显著高于无淋巴结转移组(P<0.05)。在腋淋巴结阴性的血行转移患者中,VEGF及MVD的表达显著高于腋淋巴结阳性的血行转移患者(P<0.05),而VEGF-C、Flt-4及LVD的表达却显著低于后者(P<0.05)。MVD及VEGF-C的阳性表达与乳腺癌血行转移危险密切相关。结论VEGF促进微血管生成;VEGF-C、Flt-4促进淋巴管生成,且二者有显著相关性(P<0.01);VEGF-C、Flt-4及LVD与淋巴结转移状况密切相关;VEGF及MVD与腋淋巴结阴性患者血行转移相关;MVD及VEGF-C可能是乳腺癌患者发生血行转移的危险因素。

Objective：To study the expression of VEGF, VEGF-C,Flt-4, MVD, LVD and Fh-4 positive vessel number in breast cancer, its relationship with each other and its clinical significance. Methods：S-P immunohistochemical staining was used to detect the expression of VEGF, VEGF-C, Fh-4, MVD, LVD and Flt-4 positive vessel number in 105 cases of primary breast cancer. Resuits：There was significant correlation between VEGF and MVD （ P 〈0.01 ） ; VEGF-C was significantly related to Flt-4 （ P 〈0. 01 ）, moreover, they all had significant correlation with LVD and Flt..4 positive vessel number （P 〈 0. 01, respectively）. The expression of VEGF-C,LVD and Flt-4 positive vessel number in lymph node metastasis group was significantly higher than that of no lymph node metastasis group （P 〈0. 05 ,respectively）. In the patients who had blood metastasis, the expression of VEGF and MVD in axillary-nodenegative group was significantly higher than that of axillary-node-positive group （P 〈 0. 05, respectively）, but the expression of VEGFC,LVD and Flt-4 positive vessel number was significantly lower than that of the latter （P 〈 0. 05, respectively）. The expression of MVD and VEGF-C in breast cancer was closely related to blood metastasis. Conclusion：VEGF promotes angiogenesis; VEGF-C and Flt-4 promote lymphangiogenesis, moreover, VEGF-C is significantly related to Flt-4 （P 〈 0. 01 ） ; VEGF-C,Flt-4 and LVD are closely related to lymph node metastasis; VEGF and MVD are related to blood metastasis in axillary-node-negative patients; MVD and VEGFC are probably the dangerous factors in patients who had blood metastasis.