人卵巢组织中钙结合蛋白Calbindin-d28k蛋白和基因的表达

Expression of the Calbindin-d28k Protein and Calbindin-d28k gene in Human Ovary

目的:分析钙结合蛋白Calbindin-d28k(CaBP-d28k)在人卵巢组织中的表达。方法:收集人卵巢组织标本23例,采用免疫组织化学方法和逆转录聚合酶链反应法检测CaBP-d28k在人卵巢中的蛋白及mRNA表达。结果:人卵巢中存在CaBP-d28k蛋白及其mRNA表达,CaBP-d28k蛋白定位于卵泡中的卵母细胞、颗粒细胞,以及少量的卵泡膜细胞和黄体中的粒黄体细胞和膜黄体细胞的胞浆中。卵泡各时期CaBP-d28k蛋白阳性表达强度由弱渐强依次为原始卵泡、初级卵泡、次级卵泡。结论:人卵巢中多种细胞表达钙结合蛋白CaBP-d28k,其表达随卵母细胞成熟而增强。

Objective： To investigate the expression and distribution of Calbindin-d28k （CaBP-d28k） in human ovary. Methods： The expression of the CaBP-d28k protein and the CaBP-d28k gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction （RT-PCR） methods. Results： Positive expression of the CaBP-d28k protein and the CaBP-d28k mRNA was observed in human ovary. The ovarian expression of the CaBP-d28k protein was localized in the cytoplasm ofoocytes, granulose cells, theca cells and two types of cells of corpus luteum. And increase was observed during the course of follicular development. Conclusions： Human ovarian tissue locally expresses CaBP-d28k, and its expression level increases with follicular development.