牛初乳胰岛素样生长因子的稳定性研究

Study on Stability of Bovine Colostrums Insulin-like Growth Factors

研究了牛初乳类胰岛素生长因子在不同温度、pH值、添加保护剂条件下对其降糖活性影响,并利用Balb/c3T3成纤维细胞糖代谢测定了IGFs降糖活性。结果表明:细胞培养液的葡萄糖浓度降到对照组的60%,与胰岛素相比同样具有明显降糖活性。IGFs在pH值2～8范围内活性稳定,在温度为4℃时活性最大,30%甘露醇能有效提高IGFs降糖活性。

The paper studied the activity of bovine colostrums insulin-like growth factors to reduce glucose concentration at different temperature and pH and by adding protective reagent. We tested the metabolic effect of bovine colostrums IGFs on balb/c3T3 fibroblast. The results showed 60 % glucose left in the conditioned media in the presence of IGFs compared with control, meaning that IGFs cluld reduce glucose activity with comparative ability to that of insulin. The activity to reduce glucose concentration reached its peak when temperature was 4℃ and pH was 2-8 with 30 % mannitol.