高浓度葡萄糖对培养的人视网膜色素上皮细胞细胞表面黏附分子-1的影响

Effect of high glucose on expression of intercelluar adhesion molecule-1 of cultured human retinal pigment epithelial cells

目的:研究高浓度葡萄糖对体外培养人视网膜色素上皮(RPE)细胞细胞间黏附分子-1(ICAM-1)表达的影响。方法:用免疫荧光法检测5.6mmol/L和30.0mmol/L葡萄糖作用1,2,3d后hRPE细胞表面ICAM-1表达量,MTT方法检测两种浓度葡萄糖作用下48hhRPE表面黏附白细胞的数量。结果:30.0mmol/L葡萄糖作用1,2,3d后hRPE细胞表面荧光着色密度分别为5.6mmol/L葡萄糖作用下荧光着色的2.4,3.8,4.0倍。30.0mmol/L葡萄糖作用48h后hRPE细胞表面黏附的白细胞数量是5.6mmol/L葡萄糖组的2.34倍,有统计学显著性差异(P<0.05)。结论:高糖可以促进体外培养hRPE细胞的ICAM-1表达,提示RPE细胞在高糖作用下可能参与了糖尿病视网膜病变的早期病理反应。

AIM：To investigate the effect of high glucose on expression of intercelluar adhesion molecule-1 （ICAM-1） of human retinal pigment epithelial （hRPE） cells in vitro. METHODS： The expression of ICAM-1 of hRPE cells cultured with 5.6mmol/L and 30.0mmol/L of glucose for 1, 2 and 3d was measured with immunofluorescence staining. The number of leucocytes adhering to the surface of hRPE cells cultured with the two concentrations of glucose for 48h was detected with MTT colorimetry. RESULTS： The fluorescence staining density on the surface of hRPE cells cultured with 30.0mmol/L of glucose for 1, 2 and 3d was 2.4, 3.8 and 4.0 times as that with 5.6mmol/L of glucose, respectively. The number of leucocytes adhering to the surface of hRPE cells cultured with 30.0mmol/L of glucose for 48h was 2.34 times as that with 5.6mmol/L glucose. The difference between the two groups was statistically significant （P〈0.05）. CONCLUSION： hRPE cells cultured with high glucose can enhance expression of ICAM-1 of the cells in vitro. It suggests that hRPE cells under the effect of high glucose may participate in the pathological reaction of diabetic retinopathy in the early stage.