摘要
AIM： To observe the effect of protocatechuic aldchyde on the proliferation of hepatic stellate cells （HSCs）. METHODS： Liver fibrosis was induced in rats by carbon tetrachloride （CCh）. Then normal and fibrotic drug sera were extracted from rats. The effects of protocatechuic aldchyde, raw Radix Salvia miltiorrhiza and drug sera of Salvia miltiorrhiza on HSC growth were determined by CCKoS. The protocatechuic aldchyde was separated by high performance liquid chromatography （HPLC） in a AIItima C18 column （250 mm × 4.6 mm, 5 μm） with a mobile phase of acetonitrile-4% glacial acetic acid solution （gradient elution） at the wavelength of 281 nm. RESULTS： Protocatechuic aldchyde, raw Radix Salvia miltiorrhiza and drug sera of Salvia miltiorrhiza were found to have inhibitory effects on proliferation of rat HSCs. Raw Radix Salvia miltiorrhiza had a stronger inhibitory effect than the drug sera. The fibrotic drug sera showed a higher suppressive effect than the normal drug sera （P 〈 0.05）. Protocatechuic aldchyde was found in crude materials of both Radix Salvia miltiorrhiza and its corresponding drug sera. The average recovery （n = 6） was 110.5% for raw Salvia miltiorrhiza Bge, 102% for normal drug sera and 105.2% for fibrotic drug sera. The relative standard devitation （RSD） was 0.37%, 1.96% and 1.51%, respectively （n=6）. The contents of protocatechuic aldchyde were 0.22%, 0.15% and 0.19%, respectively （n = 6） （P〈 0.05）. The RSD was 0.33%, 0.75% and 1.24% （n=6） for raw material of Radix Salvia miltiorrhiza, normal drug sera and fibrotic drug sera, respectively. The samples were stable for 6 d. CONCLUSION： Protocatechuic aldchyde can inhibit the growth of HSCs. HPLC is suitable for the determination of virtual bioactive components of Chinese herbal medicines in vitro.
瞄准：在肝的星形细胞(HSC ) 的增长上观察 protocatechuic aldchyde 的效果。方法：肝纤维变性被四氯化碳(CCl4 ) 在老鼠导致。然后正常、纤维变性的药重量的单位一从老鼠被提取。protocatechuic aldchyde，未加工的根值鼠尾草植物 miltiorrhiza 和药重量的单位的效果 HSC 生长上的 of 鼠尾草植物 miltiorrhiza 被 CCK-8 决定。protocatechuic aldchyde 被高效液相色谱法(HPLC ) 在 Alltima C18 列分开(250 公里 mult 4.6 公里， 5 microm ) 与在 281 nm 的波长的 acetonitrile-4% 冰乙酸答案(坡度洗脱) 的一个活动阶段。结果：of 鼠尾草植物 miltiorrhiza 被发现在老鼠 HSC 的增长上有禁止的效果的 Protocatechuic aldchyde，未加工的根值鼠尾草植物 miltiorrhiza 和药重量的单位。未加工的根值鼠尾草植物 miltiorrhiza 比药重量的单位有更强壮的禁止的效果一。纤维变性药重量的单位一比正常的药重量的单位显示出更高镇压的效果一(P < 0.05 ) 。Protocatechuic aldchyde 在两根值鼠尾草植物 miltiorrhiza 和它的相应的药重量的单位的粗略的材料被发现一。平均恢复(n = 6 ) 为未加工的鼠尾草植物 miltiorrhiza Bge 是 110.5% ， 102% 为正常的药重量的单位一 and 105.2% 为纤维变性药重量的单位一。相对标准 devitation (RSD ) 是 0.37% ， 1.96% 和 1.51% ，分别地(n = 6 ) 。protocatechuic aldchyde 的内容是 0.22% ， 0.15% 和 0.19% ，分别地(n = 6 )(P < 0.05 ) 。RSD 是 0.33% ， 0.75% 和 1.24%(n = 6 ) 为根值鼠尾草植物 miltiorrhiza 的原料，正常的药重量的单位一个 and 纤维变性药重量的单位一分别地。样品为 6 d 是稳定的。结论：Protocatechuic aldchyde 能禁止 HSC 的生长。HPLC 对中草药在试管内的虚拟简历有功成分的决心合适。
