摘要
从人胎肝中提取得总ｍＲＮＡ，然后通过ＲＴａｓｅ反转录得到ｃＤＮＡ，利用ＰＣＲ技术克隆到ＥＰＯ基因（全长为５２７ｂＰ），经过计算机分析已知ＥＰＯ基因保守区段的限制性内切酶位点，选择ＢｇｌⅠ和ＨｉｎｃⅡ进行酶解，确认所获得ＤＮＡ片段为ＥＰＯ基因。
Human erythropoietin gene has been cloned via reversal transcription of human fetal liver mRNA and PCR. Restriction map of PCR products with Bgl Ⅰ and Hinc Ⅱ is identical to the map produced by computer analysis of the known EPO sequence. The results confirm that the cloned PCR products is EPO gene proper.
基金
广西自然科学基金
关键词
人胎肝
EPO基因
反转录
限制性内切酶
human fetal liver
EPO gene
reverse transcription
PCR
restriction enzyme
