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苦马豆素对肝癌细胞在体内外生长的抑制作用 被引量:23

Inhibition on the growth of Swainsonine to hepatocarcinoma in vivo and in vitro
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摘要 目的研究苦马豆素(Sw)对肝癌细胞在体内外生长的抑制作用。方法采用体外抑瘤试验培养人源性肝癌HHCC细胞的方法,通过MTT法等试验测定其体外抑癌浓度;体内试验采用小鼠的鼠源性肝癌H22移植瘤法,通过对抑瘤率及病理切片的观测,探讨其抑瘤作用。结果3.84μg.ml-1的Sw体外作用72 h后,HHCC细胞的存活率明显下降(P<0.05),其24 h的IC50为0.4μg.ml-1;SW在3、61、2 mg.kg-1时,对肝癌H22实体移植瘤的抑制率分别为13.2%、28.9%、27.3%;对腹水型H22的小鼠生命延长率分别为48.40%、64.16%、58.22%,病理切片显示,用药后肿瘤组织明显出血、坏死和炎症细胞浸润。结论苦马豆素对人源性和鼠源性肝癌生长都有一定的抑制作用。 OBJECTIVE To investigate the effect of Swainsonine on hepatocarcinoma in vivo and in vitro. METHODS After being treated with Swainsonine, the inhibition concentration of swaiasonine to HHCC cells were examined by MTT assay. The models of ascetic and noumenal transplantation tumor of H22 were established,70 mice were devided randomly into five groups, three groups of which were administrated swainsonine at 3,6,12 μg·ml^-1, two control groups were administrated NS 20 mg·ml^-1 and 5 - Fu 20 mg·ml^-1 by intraperitoneal injection, respectively. The rate of inhibition and llfe prolongation were calculated and pathological sections were observed. RESULTS Swainsonine inhibited HHCC cells proliferation significantly at the concentration above 3.84μg·ml^-1 for 72 h and its IC50 was 0.4 μg·ml^-1 at 24 h. The rate of inhibition were 13.2% ,28.9% ,27.3% and life prolongation rate were 48.40% ,64.16% ,58.22% respectively when the mice were administrated with swainsonine of 3,6, 12 mg·kg^-1. The structure of tumor showed hemorrhage, necrosis and inflammatory cell infiltration. CONCLUSION Swainsonine could inhibit the growth of hepatocarcinoma of different source.
出处 《华西药学杂志》 CAS CSCD 北大核心 2006年第3期221-224,共4页 West China Journal of Pharmaceutical Sciences
关键词 苦马豆素 肝癌 HttCC细胞 %瘤细胞 MTT实验 Swainsonine Hepatocarcinoma HHCC cell H22 MTT assay
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  • 1Li B, Kawatkar SP, George S, et al. Inhibition of Golgi mannosidase Ⅱ with mannostatin A analogues: synthesis, biological evaluation, and structure - activity relationship studies [ J ]. Chembiochem, 2004,5 ( 9 ) :1220- 1227.
  • 2Gardner DR, Molyneux RJ, Ralphs MH. Analysis of Swainsonine: extraction methods, detection, and measurement in populations of locoweeds (Oxytropis spp. ) [ J ]. J Agric Food Chem, 2001,49 (10) :4573 - 4580.
  • 3杨鸣琦,曹光荣.苦马豆素的性质、分离与检测[J].动物医学进展,1997,18(1):8-13. 被引量:7
  • 4童德文,曹光荣,李绍君.甘肃棘豆中苦马豆素的分离与鉴定[J].西北农林科技大学学报(自然科学版),2001,29(3):5-8. 被引量:60
  • 5WANG Si - Wang, LI Yu - Rong, SUN Ji - Yuan, et al. Experiment study of Swainsonine's antineoplastic activity[J]. Medical Colleges of PLA,2003,18(3) : 191 - 192.
  • 6陈绍淑,赵宝玉,莫重辉,童德文,王立红,王凯,曹光荣.苦马豆素对人食道癌Eca-109细胞体外生长的抑制试验[J].动物医学进展,2003,24(3):91-92. 被引量:19
  • 7王四旺,李予蓉,孙纪元,谢艳华,王三虎,赵越平.苦马豆素的抑瘤作用[J].中国新医药,2003,2(11):27-29. 被引量:5
  • 8刘炳亚,林言箴,尹浩然,陈雪华.苦马豆素抑制胃癌生长及转移的实验研究[J].中华肿瘤杂志,1998,20(3):168-170. 被引量:46
  • 9Fiaux H, Popowycz F, Favre S, et al. Functionalized pyrrolidines inhibit alpha- mannosidase activity and growth of human glioblastoma and melanoma cells [J]. J Med Chem, 2005,48(13):4237 - 46.
  • 10Przybylo M, Litynska A, Pochec E. Different adhesion and migration properties of human HCV29 non - malignant urothelial and T24 bladder cancer cells: role of glycosylation[J]. Biochimie,2005,87(2):133 - 42.

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