伴刀豆球蛋白A与巨噬细胞膜受体结合对膜下F－actin的影响

EFFFCT OF CONA-RECEPTIOR INTERACTION ON THE ACTIN FILAMENT ASSEMBLY IN MACROPHAGE

配体与膜受体结合可启动细胞信息传递通路，激活细胞并产生生物学效应。应用共聚焦激光扫描显微术，流式细胞分光光度计，生物活性测量等技术，研究ＭＡ与巨噬细胞膜受体结合后，膜下肌动蛋白丝构筑和含量随时间变化，以及细胞热能量改变。结果是ＣｏｎＡ结合巨噬细胞膜受体后，膜下肌动蛋白多聚化加快，构筑成细胞内Ｆ－ａｃｔｉｎ立体空间网络，Ｆ－ａｃｔｉｎ含量增加具有时间相关性，细胞热能量增加。巨噬细胞内这些变化提示ＣｏｎＡ通过膜受体诱导膜下肌动蛋白多聚化和构筑过程有信息传递和激活细胞等重要作用。

Signal fornsduction folloWed by aCtiVation of the Cells could be initined by ligandscombmmg with membrane recrptors. In this paper, the changes of actin polymerization,F-actin organzation and distribution, and thermodynach in macrophage induced byConA-receptor inthection were studied by using confocal laser scanning microscope system.flow cytometry and differential scanning calorimetry.The results showed that when ConA combined with sauce recptor of macrophage, the content of F-actin was inmesed actin filamentS were meshed within the cells and the releasingheat of the Cells was also inCreased. These changes suggestal that the course of actin filamentsassembly and meshwork induce by etnA wiht play an boortant role in signal hansductionand cell activation.