RP-HPLC梯度法测定8种蛇胆中结合型胆汁酸的含量

RP-HPLC determination of eight conjugate bile acids in Snake gall of gradient elution method

目的:用 HPLC 梯度洗脱法同时测定蛇胆中牛磺胆酸(TCA)、牛磺鹅去氧胆酸(TCDCA)、牛磺去氧胆酸(TDCA)、甘氨胆酸(GCA)4种结合型胆汁酸的含量。方法:用美国 Zorbax Extend C_(18)柱,乙腈-0.15%磷酸氢二钠溶液梯度洗脱0～20min(15:85～40:60)。流速1.0mL·min^(-1),检测波长203nm。结果:TCA、TCDCA、TDCA、GCA 在进样量为2～39μg范围内线性关系良好。该方法回收率 TCA 为99.5%(RSD=0.5%),TCDCA 为100.3%(RSD=1.8%),TDCA 为100.0%(RSD=1.8%),GCA 为98.9%(RSD=2.2%)。结论:HPLC 梯度法能将蛇胆中结合型胆汁酸很好分离,方法简单,准确。

Objective： An HPLC gradient elution was studied for the determination of TCA , TCDCA, TDCA, GCA in snake gall Methods：C18 column was used with a mobile phase of acetontrile -0. 05% Na2HPO4 ,0 -20 min of （ 15：80 -40： 60） gradient elution post time was 20 min,the wavelenth of detected was set at 203 nm. Results：TCA , TCDCA, TDCA , GCA calibration curves were linear at the range of 2 - 39 μg. The average recoveryies rate TCA was 99. 5% （ RSD =0. 5% ） ,TCDCA was 100. 3% （ RSD = 1.8% ） ,TDCA was 100. 0% （ RSD = 1.8% ） ,GCA was 98. 9% （RSD = 2. 2% ）respectively. Conclusion： The method is simple and accurate. The results show that the method can be used to control quality of Snake gall.