载脂蛋白基因多态性与动脉粥样硬化的关系

THE RELATIONSHIP BETWEEN APOLIPOPROTEIN GENE POLYMORPHISMS AND ATHEROSCLEROSIS

目的：研究心肌梗塞患者载脂蛋白基因ＲＦＬＰｓ与动脉粥样硬化及高脂血症的关系。方法：用ＰＣＲ检测ａｐｏＡＩ－ＣⅢ－ＡⅣ和Ｂ１００基因上两个酶切位点的ＲＦＬＰｓ；用多元Ｌｏｇｉｓｔｉｃ回归模型对动脉粥样硬化及高脂血症进行多因素分析。结果：ａｐｏＡⅠ－ＣⅢ－ＡⅣ基因上ＳａｃⅠ酶切位点的Ｓ＋等位基因相对频率及ａｐｏＢ基因上ＸｂａＩ酶切位点的Ｘ＋等位基因相对频率在心梗组明显高于正常组（Ｐ＜０．０５）。Ｌｏｇｉｓ－ｔｉｃ回归分析表明，年龄及Ｓ＋／＋和Ｓ－／＋多态性是患高脂血症的危险因素。结论：载脂蛋白基因的遗传多态性可能在一定程度上影响体内脂质代谢。

Objective:The experiment aimed at studying the relationship between apolipoprotein gene polymorphisms and atherosclerosis and hyperlipedemia in patients of myocardial infarction.Methods:Using PCR technique to determine the RFLPs of apolipoprotein AⅠ-CⅢ-AⅣgene cluster and B100gene;and using logistic regression model to analyse the effect of multifactors on atherosclerosis and hy-perlipedemia. Results:Relative frequencies of rare S+allele of apo AI-CⅢ-AⅣ gene cluster and X+allele in apoB gene were significantly higher in patients of MI than those in controls.The results of us-ing a logistic regression model showed that after adjusting other factors,age and S+ polymorphism in apo AI-CⅢ-AⅣgene cluster were risk factors for hyperlipemia.Conclusion:RFLPs of apolipoprotein genes may be associated with susceptibility to atherosclerosis and hyperlipedemia