摘要
目的研究白细胞介素-1β(IL-1β)及其Ⅰ型受体(IL-1RI)mRNA在大鼠颈动脉体中的表达。方法原位分子杂交、免疫荧光双重染色和Western blotting。结果原位分子杂交结果显示,IL-1RI mRNA阳性信号出现在颈动脉体球细胞中;免疫荧光双重染色结果显示,IL-1β表达在颈动脉体球细胞中;Western blotting结果进一步证明,IL-1β阳性反应条带出现在18 kD处,与其分子量一致。结论大鼠颈动脉体球细胞不仅表达IL-1RI,而且也表达其配体IL-1β。
Objective To study the expressions of IL-1 receptor type Ⅰ （IL-1RI） mRNA and IL-1β protein in the rat carotid body. Methods In situ hybridization, immunofluorescence double staining and Western blotting methods were used Results The result of in situ hybridization showed that the positive signal of IL-1β mRNA was mainly located in the glomus cells of the carotid body. The result of immunofluorescence double staining showed that IL-1β protein also expressed in the glomus cells of the organ. The Western blotting proved that the IL-1β immunoreactive band appeared at 18 kD, consistent with the molecular weight of the cytokine. Conclusion The glomus cells of the rat carotid body not only express IL-1RI mRNA, but also IL-1β.
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第6期677-680,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30270441
30400156
30471663)
