荧光定量PCR技术检测NGU患者CT和UU的实验研究

EXPERIMENTAL STUDY ON DETECTION OF CT AND UU IN NGU PATIENTS USING FLUORESCENCE QUANLITATIVE PCR

目的:探讨荧光定量聚合酶链反应(FQ-PCR)检测非淋菌性尿道炎(NGU)患者沙眼衣原体(CT)和解脲支原体(UU)的临床意义。方法:采用FQ-PCR方法检测526例临床上诊断为非淋菌性尿道炎患者的沙眼衣原体(CT)和解脲支原体(UU)。结果:CT阳性率9.89%(52/526),其中男性阳性率8.97%(28/312),女性阳性率11.21%(24/214);UU阳性率28.90%(152/526),其中男性阳性率16.99%(53/312),女性阳性率46.26%(99/214);UU感染率在男女性别间有高度差异性(P<0.01),而CT无显著差异(P>0.05)。CT和UU同时检出阳性占4.18%(22/526)。定量测定显示:NGU患者CT病毒载量最高7.5×108copies/ml,最低4.0×104copies/ml;UU患者CT病毒载量最高8.5×107copies/ml,最低9.6×103copies/ml。结论:FQ-PCR方法具有简便、快速、准确、特异性强等优点,可应用于非淋菌性尿道炎的诊断、治疗及疗效观察。

Objective： To study the clinical significance of fluorescence quantitative PCR （FQ- PCR） in detecting CT and UU of NGU patients. Methods： FQ- PCR was used to detect CT and UU of 526 NGU patients. Results： The positive rate of CT was 9.89% （52/526） of all, male positive rate of CT was 8.97% （28/312） and that of female was 11.21% （24/214） ; The positive rateofUU was 28.90% （152/526） of all these, male positive rate of UU was 16.99% （53/312） and that of female was 46.26 % （99/214）. Infection rate of UU was obviously statistical different between males and females （P 〈 0.01）, but CT was not （P〉0.05）. The positive rate of CT and UU was 4.18% （22/526）. This detection of fixed quantity showed that CT of NGU patients were 7.5 × 10^8 coples/ml max and 4.0 × 10^4 copies/ml min and the ones of UU were 8.5 × 10^7 copies/ml max and 9.6 × 10^3 min. Conclusion： FQ- PCR method has advantages of simple, rapid, accuracy and strong specialty. It could be applied to clinical diagnosis, therapy and therapeutical observation of NGU patients.